›› 2012, Vol. 24 ›› Issue (5): 372-375.doi: 10.3969/j.issn.1004-616x.2012.05.013

• 肿瘤防治 • 上一篇    下一篇

先天性肺囊性腺瘤样畸形中TGF-β1 的表达及意义

王利群;蔡凤梅;周玲玲;王宗敏;王卉芳;王亚楼   

  1. ( 1. 西安市第四医院病理科，陕西 西安 710004；2. 温州医学院附属第二医院病理科，浙江 温州 325027 )
  • 收稿日期:2011-12-31 修回日期:2012-04-18 出版日期:2012-09-30 发布日期:2012-09-30
  • 通讯作者: 王宗敏

The expression and significance of TGF-β1 in congenital cystic adenomatoid malformation of the lung

WANG Li-qun;CAI Feng-mei;ZHOU Ling-ling;WANG Zong-min;WANG Hui-fang;WANG Ya-lou   

  1. (1. Department of Pathology, The Fourth Hospital of Xi’an, Xi’an 710004, Shaanxi; 2. Department of Pathology, The Second Hospital, Wenzhou Medical College, Wenzhou 325027, Zhejiang, China)
  • Received:2011-12-31 Revised:2012-04-18 Online:2012-09-30 Published:2012-09-30
  • Contact: WANG Zong-min

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摘要： 目的： 探讨转化生长因子-β1 (transforming growth factor beta 1，TGF-β1)在儿童先天性肺囊性腺瘤样畸形 (congenital cystic adenomatoid malformation of lung ，CCAM)中的表达及意义。方法：选择6月~15岁的儿童CCAM手术存档蜡块标本52例为病例组，同时以不同时期 (12~39周)人胚胎肺组织15例和正常儿童 (2月~15岁)肺组织9例作为对照组，用免疫组织化学EliVision法检测TGF-β1蛋白的表达，用原位杂交方法检测TGF-β1 mRNA的表达。结果：TGF-β1蛋白主要表达在上皮和间质细胞的细胞浆中，而TGF-β1 mRNA主要表达于支气管周围平滑肌、囊壁平滑肌及血管平滑肌中，上皮细胞未见表达。与对照组相比，TGF-β1蛋白和mRNA在CCAM病例组中的表达均明显增强，差异具有统计学意义 (P<0.05或 P<0.01)；CCAMⅠ、Ⅱ、Ⅲ型之间两两比较，TGF-β1蛋白和mRNA的表达差异均无统计学意义 (P>0.05)。结论：TGF-β1在正常胚胎肺及儿童肺组织中表达较弱，而在CCAM中表达明显增强，提示TGF-β1的过表达参与CCAM的发生发展过程，可以作为进一步研究的目标。

关键词: 转化生长因子-β1, 先天性肺囊性腺瘤样畸形, 免疫组化, 原位杂交

Abstract: OBJECTIVE: To investigate the expression of TGF-β1 in congenital cystic adenomatoid malformation of the lung (CCAM) and its role in the formation of CCAM. METHODS： The diseased group included 52 cases of congenital cystoadenomatoid malformation of lung (6 m-15 y)，9 cases of normal lungs (2 m-15 y)and 15 fetal lungs at varying gestational ages (12-39 w) as the control group. Immunohistochemical staining (EliVision) was used to detect the proteins of TGF-β1，hybridization in situ to detect the mRNA of TGF-β1 in every tissue of CCAM，normal pediatric lungs and fetal lungs. RESULTS： The protein of TGF-β1 was mainly expressed in celluar cytoplasm of pulmonary epithelial cells and mesenchymal cells. The expression of TGF-β1 mRNA located in cytoplasm of smooth muscle cells surrounding the bronchus and cysts. The expression level of TGF-β1 and TGF-β1 mRNA in CCAM groups were significantly higher than those in fetal and normal lungs (P<0.05 or P<0.01)，but not significantly different among the diseased groups (P>0.05). CONCLUSION：The overexpression of TGF-β1 may play an important role in the development of congenital cystic adenomatoid malformation，and would warrant our further investigation.

Key words: TGF-β1, congenital cystic adenomatoid malformation of lung, immunohistochemistry, hybridization in situ

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